Methods

  • Sampling (on board), filtration, fixation and storage of samples.
  • Classical chemical methods for the analysis of inorganic nutrients and elements.
  • High sensitivity methods for substrate analyses (HPLC, CHN, TOC analysis).
  • Fractionation of molecular size classes of DOM and specific dissolved substrates by ultrafiltration.
  • Methods to study the occurrence of aggregates in situ and their formation experimentally (under water photography, rolling tanks).
  • Micro- and mesocosms studies.
  • Microscopy and image analysis.
  • Flow cytometry and cell sorting.
  • Classical microbiological methods to isolate, culture and physiologically characterize bacteria.
  • Molecular methods to identify bacteria and the composition of microbial communities (Denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA fragments, fluorescence in situ hybridization, RT-PCR, quantitative real time PCR, cloning, sequencing of 16S rDNA genes, genomics, metagenomics).

Instruments

  • Light and epifluorescence microscopes
  • HPLC (amino acid, monosaccharide analysis)
  • CHN analysis
  • Ultrafiltration
  • Thermocycler
  • Real Time
  • Electrophoresis (DGGE, agarose)
  • Gel documentation
  • DNA sequencer
  • Flow cytometer (FACS ARIAIII and C6)
  • Incubation of viruses, bacteria and algal species
  • Rolling tanks for the experimental simulation of tidal cycles
  • Photographic under water documentation and image analysis of suspended aggregates